ZX‐33120‐480 | Beta Galactosidase Fluorometric Assay Kit

β-Galactosidase Assay is a method used to measure the activity of the enzyme β-galactosidase, which catalyzes the hydrolysis of β-galactosides into monosaccharides. β-galactosidase is commonly used as a reporter gene in molecular biology to monitor gene expression and cellular activity in various research applications. The typical assay involves incubating a sample containing β-galactosidase with a substrate and then measuring the product concentration, which is directly proportional to the enzyme activity. The assay can be adapted for use in various sample types, including bacterial cultures, cell lysates, or tissue homogenates. The enzyme’s activity is often assessed using synthetic substrates such as ONPG (o-nitrophenyl-β-D-galactopyranoside) or X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside).

The ZELLX® β-Galactosidase Assay Kit to quantify active β-galactosidase levels in cells transfected with plasmids that express the Lac Z gene. Lac Z is a widely used reporter gene in transfection experiments due to the robustness of its encoded enzyme, β-galactosidase, against proteolytic degradation. Its activity is straightforward to measure through the hydrolysis of fluorescein di-β-D-galactopyranoside (FDG) to fluorescein, detectable at excitation/emission wavelengths of 490/525 nm. This fluorescence is directly proportional to the amount of β-galactosidase present, allowing for precise quantification of its levels in cells expressing Lac Z from transfected plasmids.

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