Description
ZELLX® Bovine Factor Xa is a highly specific serine protease engineered for the precise and efficient cleavage of recombinant fusion proteins in protein expression and purification workflows. Derived from bovine sources and purified under stringent quality standards, this enzyme provides reliable performance with minimal nonspecific proteolysis.
Factor Xa is a key component of the blood coagulation cascade and belongs to the family of trypsin-like serine proteases. It exhibits strict sequence specificity, cleaving peptide bonds C-terminal to the sequence Ile–Glu–Gly–Arg (IEGR). This high specificity makes Bovine Factor Xa an ideal tool for removing affinity tags and fusion partners without compromising the integrity or activity of the target protein.
ZELLX® Bovine Factor Xa is optimized for use in recombinant protein processing, enabling controlled and reproducible cleavage under mild reaction conditions. Its predictable activity supports efficient downstream purification and functional studies, particularly in applications requiring native protein conformation.
Manufactured under strict quality control, ZELLX® Bovine Factor Xa is purified to remove contaminating proteases and unwanted enzymatic activities. Each batch is tested to ensure consistent activity, high purity, and reproducible cleavage performance, making it suitable for use in research, biotechnology, and protein production laboratories.
Designed for compatibility with standard cleavage buffers and purification workflows, ZELLX® Bovine Factor Xa integrates seamlessly into existing protocols, reducing optimization time and improving overall process efficiency.
ZELLX® Bovine Factor Xa delivers precision, reliability, and control; making it the enzyme of choice for researchers who demand accurate and reproducible proteolytic cleavage in protein purification workflows.
Product Specifications:
Catalog Number: ZXB-06-201
Key Applications
- Site-specific cleavage of fusion proteins containing the Factor Xa recognition sequence (IEGR)
- Removal of affinity tags following recombinant protein expression
- Protein purification and downstream functional analysis
- Structural biology and biochemical studies requiring native proteins
- High purity: ≥95% confirmed by SDS-PAGE
