ZellX® PFGE agarose is a high gel strength agarose, designed to separate large DNA fragments at lower concentrations. Small DNA molecules (less than 20 kb) can be separated by standard gel electrophoresis technique as they move in a size-dependent manner, however, DNA larger than 20-25 kb move together through the gel in a size-independent manner and appear in a gel as a single large diffuse band, and therefore, the standard gel electrophoresis technique is unable to separate very large DNA molecules effectively.
Pulsed Field Gel Electrophoresis (PFGE) is a technique used to separate large DNA molecules by applying an electric field to a gel that periodically alters direction. The various size of DNA molecules react differently to periodic altering of field direction, whereby, larger DNA fragments will be slower to realign their charge in response to alteration of field direction, while smaller pieces will react quicker. Over the course of time with the consistent altering of field directions, each DNA fragment will begin to separate more and more even at very large lengths.
Catalog Number: ZXB-06-140
CAS Number: 9012-36-6
EEO (Electroendosmosis): ≤ 0.12
Sulfate: ≤ 0.1%
Gelling Temperature: ≤ 36.5 °C
DNAse/ RNAse Activity: None detected
Gel Background: Very low
Dna Binding: Very low
PubChem Chemical ID: 11966311