Description
Product Description
ZELLX® T4 DNA Polymerase is a high-fidelity, DNA-dependent DNA polymerase derived from bacteriophage T4 and optimized for DNA end repair, blunt-end formation, and second-strand DNA synthesis. The enzyme combines dual functionality, performing both DNA polymerization and exonuclease reactions, which enables precise processing of DNA termini in molecular biology workflows.
A defining feature of T4 DNA Polymerase is its strong 3′→5′ exonuclease proofreading activity, which ensures high-fidelity DNA end processing. This activity allows efficient removal of 3′ overhangs while the polymerase activity fills in 5′ overhangs, resulting in clean, blunt-ended DNA fragments suitable for cloning, labeling, and library preparation applications.
ZELLX® T4 DNA Polymerase is supplied in a ready-to-use format at 5 U/µL, formulated in an optimized storage buffer for direct application without dilution. The enzyme delivers reliable blunting efficiency across a wide range of DNA substrates and performs consistently in both routine and advanced workflows.
Manufactured under controlled and validated production conditions, ZELLX® T4 DNA Polymerase offers excellent stability and reproducibility, ensuring dependable batch-to-batch performance. The enzyme is compatible with multiple molecular biology workflows, including blunt-end cloning, probe preparation, and second-strand cDNA synthesis, making it an essential tool for research and biotechnology laboratories.
Product Specifications:
Catalog Number: ZXB-06-204
