ZX-88103-3100 | EasyAssay ™ WST-1 Assay Kit (3100 assays)
The Water-Soluble Tetrazolium salt-1 (WST-1) assay is an optimized colorimetric, non-radioactive assay for assessing cell viability, proliferation and cytotoxicity in mammalian cells based on their cellular redox potential. Dehydrogenase enzymes in metabolically active cells reduce the yellow highly water-soluble tetrazolium salt WST-1 in the presence of an electron carrier (1-methoxy-5-methylphenazinium methyl sulfate) to the brightly orange formazan dye which can be measured at 450 nm. Since this reduction can only occur in metabolically active cells, it is considered as an indicator of cell viability. The amount of produced formazan is proportional to the number of viable cells in the sample. The detection sensitivity of this assay is higher than the other tetrazolium salts such as MTT, XTT or MTS.
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