Protocol

PROTOCOLS

 

INTRODUCTION

We recommend an organic phase extraction to extract steroids from non-liquid matrices such as dried solids or other organic matter. We use ethanol or ethyl acetate as safer alternatives to diethyl ether or methylene chloride. We recommend centrifugal vacuum devices (i.e., a SpeedVacTM) to remove the solvent completely and safely after extraction. Alternatively, the organic layer can be separated and stored for later use. We also recommend using ethanol to completely solubilize the dried extracted steroid since steroids typically have limited aqueous solubility.