ZX‐88104‐3300 | EasyAssay ™ WST‐8/CCK8 Assay Kit (3300 assays)
The Water-Soluble Tetrazolium salt-8 (WST-8) assay also known in the market as Cell Counting Kit 8 (CCK8) assay is an optimized colorimetric, non-radioactive assay for assessing cell viability, proliferation and cytotoxicity in mammalian cells based on their cellular redox potential. Dehydrogenase enzymes in metabolically active cells reduce the yellow highly water-soluble tetrazolium salt WST-8 in the presence of an electron carrier (1-methoxy-5-methylphenazinium methyl sulfate) to the brightly orange formazan dye which can be measured at 460 nm. Since this reduction can only occur in metabolically active cells, it is considered as an indicator of cell viability. The amount of produced formazan correlates with the number of viable cells in the sample. The detection sensitivity of this assay is higher than the other tetrazolium salts such as MTT, XTT, MTS or WST-1.
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