Agarose beads consist of small spherical particles of agarose which are different in diameters and concentrations and are widely used for size exclusion or affinity chromatography. Standard, cross-linked and highly cross-linked agarose beads (without ligand linking) are used in Gel Filtration Chromatography (or size Exclusion Chromatography), while, for affinity chromatography, ligands such as nitrilotriacetic acid (NTA) or iminodiacetic acid (IDA) are covalently linked to the agarose bead polymer which can be further used for separation of tagged proteins from other proteins. For decades, Agarose beads have been used for protein purification. Physical characteristics of agarose beads significantly vary between different kinds of beads and result in their different applications. Standard agarose beads (not cross-linked) are used for separation techniques whereby gravity force runs the samples through the agarose matrix. However, for high-pressure separation techniques, highly cross-linked and cross-linked beads must be chosen. Highly cross-linked and cross-linked beads are physically and chemically more stable than the standard agarose beads, hence, the higher the level of cross-linking is the more stable the matrix are. To produce Highly cross-linked and cross-linked beads, agarose beads must be treated with a particular reagent.
Standard Agarose Beads (4%), Highly Cross-linked consist of 4 percent agarose beads with size of 50-150 µm which are cross-linked. It is a resin routinely used for gel filtration and coupling affinity ligands. This agarose matrix is not pre-activated and therefore needed to activated by the end user.
Catalog Number: ZXB-06-150
Bead Geometry & Size: Spherical ~50-150 µm
Agarose %: 4%
Maximum Flow Rate at 15 cm Bed Height: ≥ 500 cm/h
Maximum Pressure at 15 cm bed Height: ≥ 150 kPa
pH Stability Working Range: Short term pH 1.8-14 / long term pH 3.8-13
Temperature Stability: Autoclavable at 121˚C for 20 min in H2O
Chemical Stability: Most commonly used aqueous and organic solutions
Antimicrobial Agent: 20% Ethanol
Storage Temperature: 2-30˚C