Description
Tris-glycine buffer is commonly used in protein electrophoresis techniques, such as sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). It provides a suitable environment for separating proteins based on their molecular weight. The specific pH and concentration of Tris and glycine in the buffer can be adjusted according to the experimental requirements. Tris-glycine buffers are typically prepared as stock solutions and diluted to the desired working concentrations. Overall, Tris-glycine buffer is valued for its stability, compatibility with many biological systems, and ability to maintain pH within a desired range during experimental procedures. ZELLX® Tris-glycine, for instance, is composed of 0.025 M Tris, 0.192 M glycine, with a pH of 8.3 at 25°C.
