Tris-Glycine SDS PH 8.3

Tris-glycine SDS, also known as Tris-glycine sodium dodecyl sulfate, is a buffer system commonly used in protein electrophoresis techniques, specifically sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). It combines the Tris-glycine buffer system with the addition of sodium dodecyl sulfate (SDS), an anionic detergent. SDS plays a critical role in SDS-PAGE by denaturing proteins and imparting a negative charge to them. It disrupts protein structures, unfolds them into linear chains, and coats them with a uniform negative charge proportional to their mass. This allows for separation based primarily on size during electrophoresis.

The Tris-glycine SDS buffer system provides the necessary pH and ionic strength conditions for efficient protein separation in SDS-PAGE. The Tris component acts as the buffering agent to maintain the desired pH range, while glycine helps establish the appropriate ionic strength and charge distribution in the gel matrix. When combined with SDS, the Tris-glycine buffer system provides a robust environment for the denaturation, separation, and visualization of proteins based on their molecular weight. It is commonly used in protein analysis, such as determining protein size, purity, and relative abundance in samples.

Tris-glycine SDS buffer systems are typically prepared as stock solutions with specific pH and concentration. Researchers can adjust the pH and concentration of Tris and glycine based on the specific requirements of their experiments. ZELLX® Tris-glycine SDS, for instance, is composed of 0.025 M Tris, 0.192 M glycine, 0.10% SDS with a pH of 8.3 at 25°C.

SDS  |  CoA