The ZellX^® One-Step Reverse Transcription Polymerase Chain Reaction (RT-PCR) Kit grants efficient cDNA synthesis and PCR in a single tube. The PCR Master Mix provided in the kit contains all the reagents (except primers and RNA template) needed for running standard PCR reactions. In addition, a separate Reverse Transcriptase mix that comprises a balanced mixture of both Reverse Transcriptase and RNase Inhibitor is provided.

RT-PCR is used to amplify double-stranded DNA from single-stranded RNA templates. In the RT step the reverse transcriptase synthesizes single-stranded DNA molecules (cDNA) complementary to the RNA template. In the first cycle of the PCR, Taq DNA polymerase synthesizes DNA molecules complementary to the cDNA, thus generating a double-stranded DNA template. During subsequent cycles, the DNA polymerase exponentially amplifies the double-stranded DNA template.