ZX-22114-250/500/1000 | Probe-based qPCR Master Mix (low cxr)
ZellX® Probe -based qPCR Master Mix (low cxr) grants efficient quantitative/real-time PCR in a single tube. The probe-based qPCR Master Mix contains all the reagents (except primers, probes and DNA template) needed for running real-time PCR reactions and is specially designed for Taqman® probe technologies (Based on the TaqMan® probe detection principle, the reporter dye (at 5’ end) and quencher (at 3’ end) hybridize on a specific region within the amplified fragment (Probe-Target Binding site). Amplification of the target gene results in cleavage of probe and release of the reporter dye (fluorophore). The intensity of detected fluorescent signal is proportional to the number of amplicons).
As an internal reference, the kit contains low concentrations of carboxy-X-rhodamine (ROX™). Being independent from the amount of DNA template, the fluorescence signal of ROX™ is not influenced by the PCR reactions, and therefore can assist in the normalization of the reporter-dye signal during data analysis. The appropriate level of ROX™ depends on the real-time PCR instrument (Contact your instrument manufacturer for details). For high levels of ROX™ use our Probe-based qPCR Master Mix (high cxr) (Cat NO. ZX-22115-250/500/1000).
Compatible with most used real-time PCR machines
Specifically designed for the TaqMan® Probe technology
Use: Highly efficient, safe and sensitive real-time PCR
Quantity: 250, 500, 1000 rxns
Stability: 2 years at -20°C
Technical Support: Customer satisfaction is very important to us. Please call us at +49(0)30 81309085 or send an email to technical[at]zellx.de with your technical questions. A member of our scientific staff will be glad to assist you.