ZX-33103-96 | Glutathione S-Transferase (GST) Activity Assay (Fluorometric)
The Glutathione S-Transferase (GST) family of isozymes are expressed in almost all tissues, and function to detoxify a wide variety of electrophilic molecules by mediating their conjugation with reduced glutathione. Due to its pivotal role in ameliorating oxidative stress, the GST activity is considered as a biomarker for arthritis, asthma, COPD, and multiple forms of cancer, and is thought to substantially contribute to the innate or acquired resistance of specific neoplasms to anticancer therapy.
The ZellX® Glutathione S-Transferase Fluorescent Activity Kit is designed to quantitatively measure the activity of GST present in a variety of samples. A GST standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. The kit utilizes a non‐fluorescent substrate of GST enzyme which covalently binds glutathione (GSH) to yield a highly fluorescent product. In brief, the sample or standard is mixed with the supplied Detection Reagent and GSH, incubated at room temperature (RT) for 30 minutes to generate the fluorescent product which is read at 460 nm with excitation at 390 nm. The activity of GST in the sample is calculated with consideration of the dilution.
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