ZX-44100-96 | Glutathione (GSH) Assay (Colorimetric)
The ZellX® Glutathione (GSH) assay kit has been designed to simultaneously quantifies the level of Glutathione (GSH), and Oxidized Glutathione (GSSG) in a variety of samples without extra steps of separation or washing. Glutathione (GSH) is a major non-protein thiol with a concentration of 0.5 – 10 mM in mammalian cells, which serves as an intracellular reducing substance to protect the cells against oxidative stress. Glutathione Peroxidase (GPx) catalyzes the reduction of hydrogen peroxide (H2O2), using reduced GSH, and produces oxidized GSH dimer (GSSG). GSH also plays a critical role in many other biological processes, such as protein and DNA synthesis, and amino acids transport.
The ZellX® Glutathione (GSH) assay kit utilizes a colorimetric substrate that reacts with the free thiol group on GSH to yield a highly colored product. The concentration of Oxidized Glutathione (GSSG) is measured by blocking any free GSH in the sample using 2-Vinylpyridine (2VP _ not supplied). The Total GSH level is determined in the samples which are not treated with 2VP. The Free GSH concentration is calculated from the difference between the Total GSH and the GSSG. The concentration of GSH can be measured either as an endpoint read of the color developed at 405 nm or as a kinetic method by measuring the rate of color development at 405 nm. A GSSG standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve.
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