ZX-44105-96 | Bicinchoninic Acid (BCA) protein Assay (Colorimetric)
Bicinchoninic acid (BCA) assay is one of the most common methods of protein determination in biochemical research. The principle of the assay relies on two reactions: first, the reduction of Cu2+ to Cu1+ in an alkaline medium by cysteine, cystine, tryptophan, tyrosine, and peptide bonds present in the protein, and second, the chelation of two molecules BCA with one Cu1+ which forms a water-soluble purple-colored complex. The purple-colored product exhibits a strong absorbance at 562 nm that is nearly linear with increasing protein concentrations over a broad working range (6-1,000 µg/mL).
The ZellX® BCA assay is designed to quantitatively measure protein concentration in a variety of samples. ZellX® BCA assay Kit, can measure the amount of protein at both low and normal concentration. Cu2+ ion is reduced to Cu1+ by protein under alkaline condition which is reacting with bicinchoninic acid to generate a purple‐colored product which is read at 560 nm, proportional to the protein amount. A Bovine Serum Albumin standard is provided to create a standard curve for the assay.
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