ZX-44105-96 | Bicinchoninic Acid (BCA) protein Assay (Colorimetric)
two reactions: first, the reduction of Cu2+ to Cu1+ in an alkaline medium by cysteine, cystine, tryptophan, tyrosine, and peptide bonds present in the protein, and second, the chelation of two molecules BCA with one Cu1+ which forms a water-soluble purple-colored complex. The purple-colored product exhibits a strong absorbance at 562nm that is nearly linear with increasing protein concentrations over a broad working range (6-1,000 µg/mL).
The ZellX® BCA assay is designed to quantitatively measure protein concentration in a variety of samples. ZellX® BCA assay Kit, can measure the amount of protein at both low and normal concentration. Cu2+ ion is reduced to Cu1+ by protein under alkaline condition which is reacting with bicinchoninic acid to generate a purple‐colored product which is read at 560 nm, proportional to the protein amount. A Bovine Serum Albumin standard is provided to create a standard curve for the assay.
|Use: Measure total protein content in samples||Download||Download|
|Sample: Serum, Plasma, Urine, Cell Lysates, and Tissue Homogenates|
|Sample/Kit: 41 in Duplicate||Cat No.||Size||Price|
|Sensitivity: 1.7 µg/mL||ZX-44105-96||96 wells||108.00 €|
|Stability: Room temperature|
|Whole Kit: Just add Samples and water!|
|Ordering: You can also order using info[at]zellx.de or FAX at +49(0)30 81309086|
|Technical Support: Customer satisfaction is very important to us. Please call us at +49(0)30 81309085 or send an email to technical[at]zellx.de with your technical questions. A member of our scientific staff will be glad to assist you.|