ZX-44105-96 | Bicinchoninic Acid (BCA) protein Assay

ZX-44105-96 | Bicinchoninic Acid (BCA) protein Assay (Colorimetric)

Bicinchoninic acid (BCA) assay is one of the most common methods of protein determination in biochemical research. The principle of the assay relies on two reactions: first, the reduction of Cu2+ to Cu1+ in an alkaline medium by cysteine, cystine, tryptophan, tyrosine, and peptide bonds present in the protein, and second, the chelation of two molecules BCA with one Cu1+ which forms a water-soluble purple-colored complex. The purple-colored product exhibits a strong absorbance at 562nm that is nearly linear with increasing protein concentrations over a broad working range (6-1,000 µg/mL).

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Use: Measure total protein content in samples Download Download
Sample: Serum, Plasma, Urine, Cell Lysates, and Tissue Homogenates
Sample/Kit: 41 in Duplicate Cat No. Size Price
Sensitivity: 1.7 µg/mL ZX-44105-96 96 wells 108.00 €
Stability: Room temperature
Whole Kit: Just add Samples and water!
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