ZX-44108-96/192 | Superoxide Dismutase (SOD) Activity Assay (Colorimetric)
Superoxide Dismutases (SODs, EC126.96.36.199) are enzymes which detoxify superoxide anions (O2-) by converting (dismutating) them into two less damaging species: oxygen (O2) and hydrogen peroxide (H2O2). The dismutation reaction occurs in nearly all cells as an important antioxidant mechanism in response to O2- exposure to protect against its toxicity. There are three major families of superoxide dismutases, which are classified based on their metal cofactor: Cu/Zn, Fe/Mn, and Ni. The major intracellular SOD is a 32-kD copper and zinc containing homodimer (Cu/Zn SOD). Abnormal activities of SODs are associated with different health disorders such as familial Amyotrophic Lateral Sclerosis (ALS), perinatal lethality, neural disorders, downs syndrome, thyroid dysfunction and cancer.
The ZellX® Superoxide Dismutase (SOD) Activity Assay Kit is designed to quantitatively measure SOD activity in a variety of samples. The kit can be used to evaluate the activity of all SOD types, including Cu/Zn, Mn, and Fe SOD types. Samples are diluted in Assay Buffer and added to the wells. The Substrate is added followed by Xanthine Oxidase Reagent and incubated at room temperature for 20 minutes. The Xanthine Oxidase generates superoxide in the presence of oxygen, which converts a colorless substrate in the Detection Reagent into a yellow colored product. The colored product is read at 450 nm. Increasing levels of SOD in the samples causes a decrease in superoxide concentration and a reduction in yellow product. The results are expressed in terms of units of SOD activity per mL (U/mL).
Use: Measure SOD Activity
Sample: Serum, Plasma, Cells, Tissues and Erythrocyte Lysates
Sample/Kit: 41 & 89 in Duplicate for 96 & 192 respectively
Sensitivity: 0.044 U/mL
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