ZX-44109-96/192 | Total Antioxidant Capacity (FRAP) Assay (Colorimetric)
Antioxidants or free radical scavengers are the molecules which prevent free radicals and other potentially toxic oxidizing species from damaging cells and organisms. The source of antioxidants are either endogenous, i.e. produced by our body, or exogenous, i.e.. obtained via nutrition. Endogenous antioxidants are classified into two subgroups of enzymatic such as dismutase, reductase, catalase, peroxidase, etc. and non-enzymatic such as glutathione, bilirubin, metallothioneins, and uric acid. However, as the amount of free radicals is often higher than the capacity of endogenous antioxidants to detoxify, it is necessary to obtain some antioxidants from external sources (vitamin A, vitamin C, and vitamin E) to balance the disequilibrium. As antioxidants are different in their reducing capacity, the Ferric Reducing Antioxidant Power (FRAP) is utilized to evaluate the reducing power of antioxidants in comparison to ascorbic acid as a standard. The antioxidant capacity of biological samples is considered as an indicator of the overall potential of body against undesired effects of reactive oxygen species (ROS) and oxidative stress-related diseases.
The ZellX® Total Antioxidant Capacity (FRAP) assay is designed to quantitatively evaluate the antioxidant status in a variety of samples. The Kit measures the capacity of both small molecule antioxidants and proteins to reduce the ferric ions (Fe3+) of FRAP Color Solution to ferrous ions (Fe2+) under acidic conditions. The reaction generates a blue-colored product which is read at 560 nm, proportional to the total antioxidant capacity. A Ferrous Chloride standard is provided to create a standard curve for the assay.
Species independent
Use: Measure FRAP in 30 minutes
Sample: Serum, Plasma, Urine, Cell Lysates, Foods, Cosmetics, etc.
Sample/Kit: 41 & 89 in Duplicate for 96 &192 respectively
Sensitivity: < 6 µM Fe2+
Stability: 4°C
| Cat No. | ZX-44109-96/192 |
| Size | 96/192 wells |
| Price | 381.00/640.00 € |
Ordering: You can also order using info[at]zellx.de or FAX at +49(0)30 81309086
Technical Support: Customer satisfaction is very important to us. Please call us at +49(0)30 81309085 or send an email to technical[at]zellx.de with your technical questions. A member of our scientific staff will be glad to assist you.