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ZX-44118 | LDH‐Cell Cytotoxicity Assay Kit

Lactate dehydrogenase (LDH) is a soluble enzyme which is present in the cytosol of a wide variety of organisms. When cells are cultured in vitro, LDH can be released into the culture medium upon the loss of membrane integrity due to cell- or compound-induced cytotoxicity. Since LDH is a stable enzyme, its presence in culture media indicates the loss of cell membrane integrity in response to damage and toxicity. LDH measurement is used to analyze cellular viability and viable cell count, estimate biomass, evaluate the cytotoxicity in cultured cells and determine the health of cells and tissues.
ZellX® LDH Cell Cytotoxicity Colorimetric Assay Kit uses the formazan dye iodonitrotetrazolium (INT) as a colorimetric indicator of enzyme activity. LDH catalyzes the oxidation of lactate to pyruvate via reduction of NAD+ to NADH and H+. The NADH subsequently reduces the INT (yellow) to the highly colored formazan (red) which can be measured at 490 nm.

Use: Detection of cellular cytotoxicity
Sample/Kit: 100/500/1000 assays
Stability: 4°C / Assay Buffer & Dye solution -20°C

Cat No. ZX-44118-100/500/1000
Size 100/500/1000 assays
Price 77.00/192.00/345.00 €
Ordering: You can also order using info[at]zellx.de or FAX at +49(0)30 81309086

Technical Support: Customer satisfaction is very important to us. Please call us at +49(0)30 81309085 or send an email to technical[at]zellx.de with your technical questions. A member of our scientific staff will be glad to assist you.