Agarose Resins Variations
ZELLX® is providing high quality agarose resins for size exclusion and affinity chromatography to cover the full range of applications:
Standard Agarose Beads (4%)
Standard Agarose Beads (4%) consist of 4 percent agarose beads with size of 50-150 µm which are not cross-linked. It is a resin routinely used for gel filtration and coupling affinity ligands. This agarose matrix is not pre-activated and therefore needed to activated by the end user.
Standard Agarose Beads (6%)
Standard Agarose Beads (6%) consist of 6 percent agarose beads with size of 50-150 µm which are not cross-linked. It is a resin routinely used for gel filtration and coupling affinity ligands. This agarose matrix is not pre-activated and therefore needed to activated by the end user.
Standard Agarose Beads (4%), Cross-linked
Standard Agarose Beads (4%), Cross-linked consist of 4 percent agarose beads with size of 50-150 µm which are cross-linked. It is a resin routinely used for gel filtration and coupling affinity ligands. This agarose matrix is not pre-activated and therefore needed to activated by the end user.
Standard Agarose Beads (6%), Cross-linked
Standard Agarose Beads (6%), Cross-linked consist of 6 percent agarose beads with size of 50-150 µm which are cross-linked. It is a resin routinely used for gel filtration and coupling affinity ligands. This agarose matrix is not pre-activated and therefore needed to activated by the end user.
Standard Agarose Beads (4%), Highly Cross-linked
Standard Agarose Beads (4%), Highly Cross-linked consist of 4 percent agarose beads with size of 50-150 µm which are cross-linked. It is a resin routinely used for gel filtration and coupling affinity ligands. This agarose matrix is not pre-activated and therefore needed to activated by the end user.
Standard Agarose Beads (6%), Highly Cross-linked
Standard Agarose Beads (6%), Highly Cross-linked consist of 6 percent agarose beads with size of 50-150 µm which are cross-linked. It is a resin routinely used for gel filtration and coupling affinity ligands. This agarose matrix is not pre-activated and therefore needed to activated by the end user.
Nickel NTA Agarose Beads
Nickel-NTA Agarose beads consist of cross-linked agarose beads with size of 50-150 µm which loaded with Nickel (Ni 2+) via Nitrilotriacetic acid (NTA) linkage with ≥50 mg/ml binding capacity. This resin matrix works well with the His-Tag Buffer and used for purification of from a variety of expression systems such as baculovirus, yeast, mammalian and bacterial cells.
Glutathione Agarose Resin
Glutathione Agarose Resin consists of not cross-linked agarose beads with size of 50-150 µm which covalently linked to glutathione via sulfur atom with ≥ 8 mg recombinant GST / ml binding capacity. This resin used in affinity purification of glutathione-S-transferase (GST) and GST fusion proteins. This matrix can be used in a one-step purification method and allows rapid, mild and highly selective purifications of proteins containing glutathione binding sequences. Buffers containing reduced glutathione can be used to displace Bounded GST-fusion proteins.
Glyoxal Low Density Agarose Resin (6%) Cross-linked
Glyoxal Low Density Agarose Resin (6%) Cross-linked consist of 6 percent agarose beads with size of 50-150 µm that allow a covalent binding of agarose to lysine amino groups. The low content of aldehydes groups (15-25 µmoles Glyoxal /ml gel) in this resin provides a very good option to conjugate affinity ligands without distorting the target ligand and hence improves the stability of the ligand. Glyoxal Low Density Agarose Resin (6%) Cross-linked has the Coupling Capacity of 5-10 (mg bsa/ml gel) and is ready to use (no activation needed).
Glyoxal High Density Agarose Resin (6%) Cross-linked
Glyoxal High Density Agarose Resin (6%) Cross-linked consist of 6 percent agarose beads with size of 50-150 µm that allow a covalent binding of agarose to lysine amino groups. The high content of aldehydes groups (40-60 µmoles Glyoxal /ml gel) in this resin provides a very good option to conjugate affinity ligands without distorting the target ligand and hence improves the stability of the ligand. Glyoxal High Density Agarose Resin (6%) Cross-linked has the Coupling Capacity of 20-25 (mg bsa/ml gel) and is ready to use (no activation needed).
Glyoxal Low Density Agarose Resin (4%) Cross-linked
Glyoxal Low Density Agarose Resin (4%) Cross-linked consist of 4 percent agarose beads with size of 50-150 µm that allow a covalent binding of agarose to lysine amino groups. The low content of aldehydes groups (15-25 µmoles Glyoxal /ml gel) in this resin provides a very good option to conjugate affinity ligands without distorting the target ligand and hence improves the stability of the ligand. Glyoxal Low Density Agarose Resin (4%) Cross-linked has the Coupling Capacity of 5-10 (mg bsa/ml gel) and is ready to use (no activation needed).
Glyoxal High Density Agarose Resin (4%) Cross-linked
Glyoxal High Density Agarose Resin (4%) Cross-linked consist of 4 percent agarose beads with size of 50-150 µm that allow a covalent binding of agarose to lysine amino groups. The high content of aldehydes groups (40-60 µmoles Glyoxal /ml gel) in this resin provides a very good option to conjugate affinity ligands without distorting the target ligand and hence improves the stability of the ligand. Glyoxal High Density Agarose Resin (4%) Cross-linked has the Coupling Capacity of 15-20 (mg bsa/ml gel) and is ready to use (no activation needed).
Protein G Agarose Beads (4%) Highly Cross-linked
Protein G Agarose Beads (4%) Highly Cross-linked consist of 4 percent highly cross-linked agarose beads with size of 50-150 µm which covalently bound to rProtein G. This resin provides a very stable bond that greatly minimize leakage of the Protein G allowing for reuse of the affinity resin in several purification steps. It works well in High Flow conditions and used to purify classes, subclasses and fragments of immunoglobulins from cell culture media and other biological fluids.
Protein A Agarose Beads (4%) Cross-linked
Protein A Agarose Beads (4%) Cross-linked consist of 4 percent cross-linked agarose beads with size of 50-150 µm which covalently bound to rProtein A. This resin provides a very stable bond that greatly minimize leakage of the Protein A allowing for reuse of the affinity resin in several purification steps. Protein A Agarose Beads (4%) Cross-linked has the Binding Capacity of 25 (mg Human IgG/ml resin) and is good for the purification of a wide range of Immunoglobulins from different mammalian species.
Streptavidin Agarose Resin highly cross-linked
Streptavidin Agarose Resin consists of highly cross-linked agarose beads with size of 50-150 µm which covalently linked to Recombinant streptavidin (a tetrameric protein containing four biotin-binding sites) with free biotin binding capacity of >120 nmol/ml. This resin is used for affinity chromatography purifications of biotinylated proteins from non-biotinylated proteins, including but not limited to affinity purification of antigens when used with biotinylated antibodies.