ZX-22117-250/500/1000 | Hot Start Taq DNA Polymerase Master Mix
ZellX® Hot Start Taq DNA Polymerase Master Mix is an optimized ready-to-use master mix that contains all PCR reaction reagents (except primers and DNA template) including dNTPs, stabilizer, MgCl2 and hot start Taq DNA polymerase needed for running PCR reactions. ZellX® Hot start Taq DNA polymerase is a Taq DNA polymerase with a strong 5’→3’ polymerase activity and a weak 5’→3’ exonuclease activity but no 3’→5’ exonuclease activity (proofreading); it bounds to a proprietary monoclonal antibody that blocks polymerase activity until inactivation occurs. The heat labile antibodies are rapidly inactivated by raising the temperature to 95-97°C for 4 minutes. This prevents or minimizes primer-dimer and nonspecific product amplification. containing extra nucleotides (preferentially adenine) without template at 3´ends results in 3´overhangs PCR fragments which allows the popular TA or GC cloning. The ZellX® Hot Start Taq DNA Polymerase Master Mix formulation saves time and eliminates or decreases the risk of error and contamination due to a reduced number of pipetting steps.
Powerful (amplification of targets up to 5 kb), Inactive at Room Temperature, High specificity (minimizes unspecific amplification), Efficient (prevents or minimizes primer-dimer and nonspecific products)
Use: Real time PCR | RT-PCR and quantitative RT-PCR | Genotyping with Taqman probes | PCR fragments amplification for TA or GC cloning | Amplification from a limited DNA template or low copy number genes
Quantity: 250, 500, 1000 rxns
Stability: 2 years at -20°C
Technical Support: Customer satisfaction is very important to us. Please call us at +49(0)30 81309085 or send an email to technical[at]zellx.de with your technical questions. A member of our scientific staff will be glad to assist you.