ZX-33100-96/480 | Glutathione (GSH) Assay (Fluorometric)
Glutathione (GSH) is a major non-protein thiol with a concentration of 0.5 – 10 mM in mammalian cells, which serves as an intracellular reducing substance to protect the cells against oxidative stress. Glutathione Peroxidase (GPx) catalyzes the reduction of hydrogen peroxide (H2O2), using reduced GSH, and produces oxidized GSH dimer (GSSG).GSH plays a critical role in many other biological processes, such as protein and DNA synthesis, and amino acids transport.
(GSH), and oxidized Glutathione (GSSG) in a variety of samples without extra steps of separation or washing. The assay utilizes a Fluorescent Detection reagent which covalently binds to the free thiol group on GSH yielding a highly fluorescent product. Free GSH is read first after 15 minutes, followed by addition of a reaction mixture that converts all the GSSG into free GSH, which then reacts with the excess Fluorescent Detection reagent to yield the signal related to Total GSH content. The total concentration of GSH generated in the sample is calculated from the generated signal. The concentration of GSH can be measured at 510 nm with the excitation at 390 nm after 15 min incubation of Fluorescent Detection Reagent with sample/standard. A GSSG standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve.
Use: Measure Free and Total Glutathione in the same well in 30 minutes
Sample: Lysate, Whole Blood, RBCs, Serum, Plasma, Urine, and Tissue
Sample/Kit: 39 or 231 in Duplicate
Sensitivity: 45 nM for Free GSH and 48 nM for Total GSH
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